IL1A Protein

Produktnummer ABIN2018030

Dieses Recombinant IL1A-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für IL1A Protein (ABIN2018030)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Reinheit: > 97 % by SDS-PAGE and HPLC analyses.

Produktdetails

Sequenz: SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS

Produktmerkmale: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/mL, corresponding to a specific activity of > 5.0 x 10^8 IU/mg.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1 EU/μg of rMuIL-1μ as determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.

Antigendetails

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: Interleukin-1 alpha

Hintergrund: Interleukin-1 alpha (IL-1a) is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1a and IL-1b binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1b is a secreted cytokine, IL-1a is predominantly a cell-associated cytokine.
Synonyms: Interleukin-1 Alpha, Mouse,

Molekulargewicht: 17.9 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints