IGF1 Protein (AA 49-118)

Produktnummer ABIN2017987

Produktdetails

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 49-118

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Produktmerkmale: ED50 < 10 ng/mL, measured by a cell proliferation assay using FDCP-1 cells, corresponding to a specific activity of > 1x10^5 units/mg.
AA 49-118, expressed with an N-terminal Met.

Reinheit: > 95 % as analyzed by SDS-PAGE and HPLC.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant rat Insulin-like Growth Factor I (rrIGF-I) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrIGF-I remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: Insulin-Like Growth Factor I (IGF-I) (IGF1 Produkte)

Hintergrund: Insulin-like Growth Factor I (IGF-I) is a single chain 7 kDa growth-promoting polypeptide originally identified as somatomedin-c. It belongs to the IGF family of peptides, which also includes IGF-II and insulin. The gene expression of IGF-I is mainly regulated by Growth Hormone, and IGF-I executes its functions via signaling through transmembrane tyrosine receptors(IGF Receptors). Most circulating IFG-I is associated with the IGF Binding Protein 3 (IGFBP-3), and the IGFBPs may inhibit the actions of IGFs by competing against the IGF Receptors. IGF-I is active in post-natal and adult animals, and is crucial for somatic growth, as IGF-I null mice show marked retardation in utero. IGF-I is involved in the carcinogenesis, and related to the prostate cancer as well. Recombinant rat Insulin-like Growth Factor I (rrIGF-I) produced in E. coli is a single non-glycosylated polypeptide chain containing 71 amino acids. A fully biologically active molecule, rrIGF-I has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE.
Synonyms: Somatomedin C, IGF-I, IGFIA, IGF1

Molekulargewicht: 7.8 kDa, observed by reducing SDS-PAGE.

UniProt: P08025

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation