EGF Protein (AA 977-1029)

Produktnummer ABIN2017757

Recombinant EGF-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für EGF Protein (AA 977-1029) (ABIN2017757)

Target: EGF (Epidermal Growth Factor (EGF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % by SDS-PAGE and HPLC analysis.

Produktdetails

Proteineigenschaft: AA 977-1029

Produktmerkmale: ED50 < 0.1 ng/mL, measured by a cell proliferation assay using BALB/c 3T3 cells, corresponding to a specific activity of > 1.0x 10^7 units/mg.
AA 977-1029, expressed with an N-terminal Met.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant mouse Epidermal Growth Factor (rmEGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmEGF should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: EGF (Epidermal Growth Factor (EGF))

Hintergrund: Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor).Recombinant mouse Epidermal Growth Factor (rmEGF) produced in E. coli is a single non-glycosylated polypeptide chain containing 54 amino acids. A fully biologically active molecule, rmEGF is obtained by proprietary chromatographic techniques with a molecular mass of 6.2 kDa analyzed by reducing SDS-PAGE.
Synonyms: Epidermal Growth Factor, Urogastrone, URG

Molekulargewicht: 6.2 kDa, observed by reducing SDS-PAGE.

UniProt: P01132

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation