EPO Protein

Produktnummer ABIN1305139

Recombinant EPO-Protein exprimiert in Escherichia coli (E. coli) und wurde in 6+ Publikationen erwähnt.

Kurzübersicht für EPO Protein (ABIN1305139)

Target: EPO (Erythropoietin (EPO))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func)

Reinheit: ≥ 95 %

Produktdetails

Marke: BD Pharmingen™

Spezifität: Biological Activity Measured using TF-1 indicator cells
Specific Activity: 0.1 - 1.0 x 10^8 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation).
ED50: 0.1 - 1.0 ng/ml
Observed linear dose response range: >100 fold

Produktmerkmale: Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, and containing 1 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant mouse EPO is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law.

Aufreinigung: The recombinant mouse EPO is > 95 % pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: The endotoxin level is ≤ 0.1 ng per µg of mouse EPO, as measured in a chromogenic LAL assay.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

BD Pharmingen™ Mouse Erythropoietin Recombinant Protein - Reactivity Ms

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, and containing 1 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.

Konservierungsmittel: Azide free

Handhabung: Rapidly thaw and quick-spin product prior to use. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Referenzen

Nagao, Suga, Okano, Masuda, Narita, Ikura, Sasaki: "Nucleotide sequence of rat erythropoietin." in: Biochimica et biophysica acta, Vol. 1171, Issue 1, pp. 99-102, (1992) (PubMed).

Krantz: "Erythropoietin." in: Blood, Vol. 77, Issue 3, pp. 419-34, (1991) (PubMed).

Kitamura, Tange, Terasawa, Chiba, Kuwaki, Miyagawa, Piao, Miyazono, Urabe, Takaku: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, (1989) (PubMed).

McDonald, Lin, Goldwasser: "Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene." in: Molecular and cellular biology, Vol. 6, Issue 3, pp. 842-8, (1986) (PubMed).

Shoemaker, Mitsock: "Murine erythropoietin gene: cloning, expression, and human gene homology." in: Molecular and cellular biology, Vol. 6, Issue 3, pp. 849-58, (1986) (PubMed).

JACOBSON, GOLDWASSER, FRIED, PLZAK: "Role of the kidney in erythropoiesis." in: Nature, Vol. 179, Issue 4560, pp. 633-4, (1957) (PubMed).

Antigendetails

Target: EPO (Erythropoietin (EPO))

Andere Bezeichnung: EPO

Substanzklasse: Hormone

Hintergrund: Mouse erythropoietin (EPO) is a 30 kD heavily glycosylated protein containing 166 amino acids. The carbohydrate residues compose approximately 30 % of the molecule by weight.3 It shares 80 % and 95 % homology with human and rat EPO, respectively. EPO functions as the survival and proliferation factors of late erythroid progenitor cells (CFU-E). In adult mammals, EPO is synthesized almost exclusively in the kidneys.

Pathways: JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity