IL-8 Protein (AA 23-99)

Produktnummer ABIN1096965

Dieses Recombinant IL-8-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für IL-8 Protein (AA 23-99) (ABIN1096965)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 23-99

Verwendungszweck: Recombinant Human Interleukin-8/IL-8 (Ala23-Ser99)

Sequenz: AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS

Produktmerkmale: Recombinant Human Interleukin-8/IL-8 (3-79) produced in E. coli is a single non-glycosylated polypeptide chain containing 77 amino acids with a molecular mass of 8,904 Daltons.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Kommentare:

Biological activity: Recombinant IL-8 (3-79) is fully biologically active when compared to standards.ED50 is less than 2 ng/ml as determined by its chemotaxis of hCXCR-2 transfected mouse BaF/3 cells.Specific Activity of 5.0 x 105 IU/mg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 months

Antigendetails

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Andere Bezeichnung: Il-8-77

Hintergrund: Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states.
Alternative Names: Interleukin-8, IL-8, C-X-C Motif Chemokine 8, Emoctakin, Granulocyte Chemotactic Protein 1, GCP-1, Monocyte-Derived Neutrophil Chemotactic Factor, MDNCF, Monocyte-Derived Neutrophil-Activating Peptide, MONAP, Neutrophil-Activating Protein 1, NAP-1, Protei

Molekulargewicht: 8.9 kDa

UniProt: P10145

Pathways: TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagie