IGF1 ELISA Kit

Produktnummer ABIN930359

Produktdetails IGF1 ELISA Kit

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reaktivität: Human

Methodentyp: Sandwich ELISA

Applikation: ELISA

Produktmerkmale: ELISA kit for the detection of Human IGF1 in the research laboratory
Alternative Names: IGF1 ELISA kit, Insulin-like Growth Factor 1 ELISA kit, Human IGF1 ELISA kit, IGF-1 ELISA kit

Aufreinigung: Immunoaffinity chromatography

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end user

Testdurchführung:

This ELISA for IGFI E20 is a socalled SandwichAssay using two specific and high affinity antibodies. The IGFI in the samples binds to the first antibody coated on the microtiterplate, the second specific antiIGFIantibody binds inturn to the immobilised IGFI. The second antibody is biotinylated, the subsequently incubated StreptavidinperoxidaseEnzyme Conjugate will bind to it, and thus in the final substrate incubation step colour development will be catalysed quantitatively depending on the IGFIlevel of the samples. The Standards of the ELISA E20 are preparedfrom recombinant IGFI in concentrations of 1, 5, 15, 30 and 50 ng/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C.

Details zu IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: IGF1 (IGF1 Produkte)

Hintergrund: Insulin-like growth factors (IGF) I and II play a pivotal role in regulating the proliferation, differentiation and specific functions of many cell types. IGFI is identical with Somatomedin C (SmC) and has a molecular weight of 7649 Da. Its major regulators are growth hormone (GH) and nutrition, although its production in specific tissues is affected by a multitude of tropic hormones and other peptide growth factors. In contrast to many other peptide hormones, IGFs are avidly bound to specific binding proteins (IGFBP). The seven classes of IGFBPs which are known at present either bind IGFI and IGFII with similar affinities or show a preference for IGFII.
Synonyms: IGF1 ELISA kit, Insulin-like Growth Factor 1 ELISA kit.

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation