IL1A ELISA Kit

Produktnummer ABIN921060

Das Maus IL1A ELISA Kit (ABIN921060) ist ein Colorimetric ELISA Kit zur Detektion von Maus IL1A. Das Kit wurde in 6 Publikationen zitiert.

Kurzübersicht für IL1A ELISA Kit (ABIN921060)

Target: IL1A (Interleukin 1 alpha (IL1A))

Bindungsspezifität: AA 115-270

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 4.7 pg/mL - 300 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails IL1A ELISA Kit

Untere Nachweisgrenze: 4.7 pg/mL

Verwendungszweck: Sandwich Mouse IL-1 Alpha ELISA Kit to quantitate Mouse Il1a in cell culture supernatants and serum.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: S115-S270

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 6.7%, 4.3%, 4.4%
Inter-Assay Precision (CV%): 7.4%, 5.5%, 4.7%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für IL1A ELISA Kit (ABIN921060)

Li, Huang, Shen: "The association of oxidative stress and pro-inflammatory cytokines in diabetic patients with hyperglycemic crisis." in: Journal of diabetes and its complications, Vol. 28, Issue 5, pp. 662-6, (2014) (PubMed).

Lin, Liu, Fang, Yu, Xiong, Yuan: "Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 68, pp. 290-6, (2014) (PubMed).

Pan, Wang: "Synergistic interaction between choline and aspirin against acute inflammation induced by carrageenan and lipopolysaccharide." in: International immunopharmacology, Vol. 20, Issue 1, pp. 229-37, (2014) (PubMed).

Liu, Yang, Guo, Zhu, Shi, Wang, Qi, Guo, Ye, Yang, Liang: "Functional blockage of EMMPRIN ameliorates atherosclerosis in apolipoprotein E-deficient mice." in: International journal of cardiology, Vol. 168, Issue 4, pp. 3248-53, (2013) (PubMed).

Duan, Liao, Huang, Nie, Wu: "HSV-1 miR-H6 inhibits HSV-1 replication and IL-6 expression in human corneal epithelial cells in vitro." in: Clinical & developmental immunology, Vol. 2012, pp. 192791, (2012) (PubMed).

Kouadir, Yang, Tan, Shi, Lu, Zhang, Yin, Zhou, Zhao: "CD36 participates in PrP(106-126)-induced activation of microglia." in: PLoS ONE, Vol. 7, Issue 1, pp. e30756, (2012) (PubMed).

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL1A

Hintergrund:

Background: Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin (IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18 kDa.

Gene Full Name: interleukin 1 alpha

Gen-ID: 16175

UniProt: P01582

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints