IL1A ELISA Kit

Produktnummer ABIN8126246

Maus IL1A ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus IL1A.

Kurzübersicht für IL1A ELISA Kit (ABIN8126246)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL1A ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: This assay employs an antibody specific for Anti-mouse IL-1a coated on a 96-well plate.

Analytische Methode: Quantitative

Produktmerkmale: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-1a concentrations in serum, plasma.  Detection Range: 15.6- 1000 pg/mL Sensitivity: < 9.4 pg / ml

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-mouse IL-1a coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: Please refer to the Manual

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL-1 Alpha (Interleukin-1 Alpha)

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints