TLR4 CLIA Kit

Produktnummer ABIN8088074

Dieses Chemiluminescent ELISA kit dient der quantitativen Messung von Maus TLR4.

Kurzübersicht für TLR4 CLIA Kit (ABIN8088074)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 13.7 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Produktdetails TLR4 CLIA Kit

Untere Nachweisgrenze: 13.7 pg/mL

Verwendungszweck: Mouse Toll Like Receptor 4 (TLR4) CLIA Kit for detecting TLR4 in tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 5.5 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Andere Bezeichnung: Toll Like Receptor 4

Hintergrund: Gene Aliases: CD284, TOLL, HToll

Gen-ID: 21898

UniProt: Q9QUK6

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteine