BRAF ELISA Kit

Produktnummer ABIN8087086

Human und Maus BRAF ELISA Kit, Colorimetric Assay für die Quantifizierung von Human und Maus BRAF.

Kurzübersicht für BRAF ELISA Kit (ABIN8087086)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Bindungsspezifität: pSer446

Reaktivität: Human, Maus

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Culture Cells

Produktdetails BRAF ELISA Kit

Verwendungszweck: Phospho-B-RAF (Ser446) Colorimetric Cell-Based ELISA Kit for detecting Phospho-B-RAF (Ser446) in researchers plate their cell line of choice.

Analytische Methode: Qualitative

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Testdauer: 4 - 5 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 6 months

Details zu BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Andere Bezeichnung: B-RAF

Hintergrund: Gene Aliases: B-RAF, B-RAF proto-oncogene serine/threonine-protein kinase, B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, c-RMIL, kinase B-Raf, p94, RAFB1, RMIL, RMIL serine/threonine-protein kinase, v-Raf murine sarcoma viral oncogene homolog B1

Gen-ID: 673

UniProt: P15056

Pathways: MAPK Signalweg, RTK Signalweg, Neurotrophin Signalübertragung, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagie