PDK4 ELISA Kit

Produktnummer ABIN8086058

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus PDK4.

Kurzübersicht für PDK4 ELISA Kit (ABIN8086058)

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Produktdetails PDK4 ELISA Kit

Untere Nachweisgrenze: 0.312 ng/mL

Verwendungszweck: Mouse Pyruvate Dehydrogenase Kinase 4 (PDK4) ELISA Kit for detecting PDK4 in serum, plasma, tissue homogenates

Analytische Methode: Quantitative

Sensitivität: 0.127 ng/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Details zu PDK4

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Andere Bezeichnung: Pyruvate Dehydrogenase Kinase 4

Hintergrund: Gene Aliases: [Pyruvate dehydrogenase (acetyl-transferring) ] kinase isozyme 4, mitochondrial, Pyruvate Dehydrogenase Kinase Isozyme 4

Gen-ID: 27273

UniProt: O70571

Pathways: PI3K-Akt Signalweg, Hedgehog Signalweg, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt