PDK4 ELISA Kit
Kurzübersicht für PDK4 ELISA Kit (ABIN8086057)
Target
Alle PDK4 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 0.156 ng/mL
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Verwendungszweck
- Human Pyruvate Dehydrogenase Kinase 4 (PDK4) ELISA Kit for detecting PDK4 in tissue homogenates, cell lysates
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Analytische Methode
- Quantitative
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Sensitivität
- 0.058 ng/mL
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Bestandteile
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Benötigtes Material
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) ELISA Kit
PDK4 Reaktivität: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) ELISA KitPDK4 Reaktivität: Maus Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) ELISA KitPDK4 Reaktivität: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Kommentare
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Probenmenge
- 100 μL
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Testdauer
- 3 h
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Plattentyp
- Pre-coated
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Testpräzision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- 4°C for one month, Long term: see manual.
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Haltbarkeit
- 12 months
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- PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))
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Andere Bezeichnung
- Pyruvate Dehydrogenase Kinase 4
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Hintergrund
- Gene Aliases: [Pyruvate dehydrogenase (acetyl-transferring) ] kinase isozyme 4, mitochondrial, Pyruvate Dehydrogenase Kinase Isozyme 4
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Gen-ID
- 5166
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UniProt
- Q16654
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Pathways
- PI3K-Akt Signalweg, Hedgehog Signalweg, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt
Target Alle PDK4 ELISA Kits anzeigen
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