IGF1 CLIA Kit

Produktnummer ABIN8081200

Maus IGF1 ELISA Kit, Chemiluminescent Assay für die Quantifizierung von Maus IGF1.

Kurzübersicht für IGF1 CLIA Kit (ABIN8081200)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 27.4 pg/mL - 20000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails IGF1 CLIA Kit

Untere Nachweisgrenze: 27.4 pg/mL

Verwendungszweck: Mouse Insulin Like Growth Factor 1 (IGF1) CLIA Kit for detecting IGF1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytische Methode: Quantitative

Sensitivität: 9.7 pg/mL

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Testpräzision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 12 months

Antigendetails

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: Insulin Like Growth Factor 1

Hintergrund: Gene Aliases: IGFI, IGF1A, IBP1, MGF, Somatomedin C, Mechano Growth Gactor

Gen-ID: 16000

UniProt: Q8CAR0

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation