E2F1 ELISA Kit

Produktnummer ABIN8078345

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human und Maus E2F1.

Kurzübersicht für E2F1 ELISA Kit (ABIN8078345)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Bindungsspezifität: pThr433

Reaktivität: Human, Maus

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Culture Cells

Produktdetails E2F1 ELISA Kit

Verwendungszweck: Phospho-E2F1 (Thr433) Colorimetric Cell-Based ELISA Kit for detecting Phospho-E2F1 (Thr433) in researchers plate their cell line of choice.

Analytische Methode: Qualitative

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Testdauer: 4 - 5 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 6 months

Details zu E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Andere Bezeichnung: E2F1

Hintergrund: Gene Aliases: E2F-1, PBR3, PRB-binding protein E2F-1, RBAP-1, RBBP-3, RBBP3, Retinoblastoma binding protein 3, Retinoblastoma-associated protein 1, Transcription factor E2F1

Gen-ID: 1869

UniProt: Q01094

Pathways: p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie