CDK1 ELISA Kit

Produktnummer ABIN8077733

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human, Ratte und Maus CDK1.

Kurzübersicht für CDK1 ELISA Kit (ABIN8077733)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Bindungsspezifität: pThr14

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Culture Cells

Produktdetails CDK1 ELISA Kit

Verwendungszweck: Phospho-CDK1/CDC2 (Thr14) Colorimetric Cell-Based ELISA Kit for detecting Phospho-CDK1/CDC2 (Thr14) in researchers plate their cell line of choice.

Analytische Methode: Qualitative

Bestandteile:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Benötigtes Material:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Testdauer: 4 - 5 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C for one month, Long term: see manual.

Haltbarkeit: 6 months

Details zu CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: CDK1/CDC2

Hintergrund: Gene Aliases: Cell division protein kinase 2, kinase Cdk2, p33 protein kinase

Gen-ID: 1017

UniProt: P06493

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA