Src ELISA Kit

Produktnummer ABIN7830896

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Src.

Kurzübersicht für Src ELISA Kit (ABIN7830896)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Src ELISA Kit

Verwendungszweck: SRC (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SRC expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Andere Bezeichnung: SRC

Hintergrund:

Synonyms: ASV|SRC1|c-SRC|p60-Src

Gene Description: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Gen-ID: 6714

Pathways: JAK-STAT Signalweg, Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling