Paxillin ELISA Kit

Produktnummer ABIN7830869

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Paxillin.

Kurzübersicht für Paxillin ELISA Kit (ABIN7830869)

Target: Paxillin (PXN)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Paxillin ELISA Kit

Verwendungszweck: PXN (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PXN expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Paxillin

Target: Paxillin (PXN)

Andere Bezeichnung: PXN

Hintergrund:

Synonyms: FLJ16691

Gene Description: paxillin

Gen-ID: 5829

Pathways: MAPK Signalweg, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling