JAK3 ELISA Kit

Produktnummer ABIN7830714

Das Human JAK3 ELISA Kit (ABIN7830714) ist ein Colorimetric ELISA Kit zur Detektion von Human JAK3.

Kurzübersicht für JAK3 ELISA Kit (ABIN7830714)

Target: JAK3 (Janus Kinase 3 (JAK3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails JAK3 ELISA Kit

Verwendungszweck: JAK3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of JAK3 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu JAK3

Target: JAK3 (Janus Kinase 3 (JAK3))

Andere Bezeichnung: JAK3

Hintergrund:

Synonyms: JAK-3|JAK3_HUMAN|JAKL|L-JAK|LJAK

Gene Description: Janus kinase 3 (a protein tyrosine kinase, leukocyte)

Gen-ID: 3718

UniProt: P52333

Pathways: JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response