JAK2 ELISA Kit

Produktnummer ABIN7830713

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human JAK2.

Kurzübersicht für JAK2 ELISA Kit (ABIN7830713)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails JAK2 ELISA Kit

Verwendungszweck: JAK2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of JAK2 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Andere Bezeichnung: JAK2

Hintergrund:

Synonyms: JTK10

Gene Description: Janus kinase 2 (a protein tyrosine kinase)

Gen-ID: 3717

UniProt: O60674

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response