Insulin Receptor ELISA Kit

Produktnummer ABIN7830705

Das Human Insulin Receptor ELISA Kit (ABIN7830705) ist ein Colorimetric ELISA Kit zur Detektion von Human Insulin Receptor.

Kurzübersicht für Insulin Receptor ELISA Kit (ABIN7830705)

Target: Insulin Receptor (INSR)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Insulin Receptor ELISA Kit

Verwendungszweck: INSR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of INSR expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Insulin Receptor

Target: Insulin Receptor (INSR)

Andere Bezeichnung: INSR

Hintergrund:

Synonyms: CD220|HHF5

Gene Description: insulin receptor

Gen-ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signalweg, RTK Signalweg, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity