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Insulin Receptor ELISA Kit

INSR Reaktivität: Human pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN4886147
  • Target Alle Insulin Receptor (INSR) ELISA Kits anzeigen
    Insulin Receptor (INSR)
    Bindungsspezifität
    pTyr
    Reaktivität
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phosphotyrosine Insulin R ELISA Kit. This assay semi-quantitatively measures phosphotyrosine Insulin R in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-Insulin R.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle Insulin Receptor (INSR) ELISA Kits anzeigen
    Insulin Receptor (INSR)
    Andere Bezeichnung
    Insulin R (INSR Produkte)
    Synonyme
    CD220 ELISA Kit, HHF5 ELISA Kit, 4932439J01Rik ELISA Kit, D630014A15Rik ELISA Kit, IR ELISA Kit, IR-A ELISA Kit, IR-B ELISA Kit, 18402 ELISA Kit, CG18402 ELISA Kit, DIHR ELISA Kit, DILR ELISA Kit, DIR ELISA Kit, DIRH ELISA Kit, DIRbeta ELISA Kit, DInR ELISA Kit, DInr ELISA Kit, Dir-a ELISA Kit, Dir-b ELISA Kit, Dmel\\CG18402 ELISA Kit, INR ELISA Kit, INS ELISA Kit, Inr ELISA Kit, Inr-alpha ELISA Kit, Inr-beta ELISA Kit, InsR ELISA Kit, dINR ELISA Kit, dIR ELISA Kit, dIRH ELISA Kit, dInR ELISA Kit, dInr ELISA Kit, dInsR ELISA Kit, dinr ELISA Kit, dir ELISA Kit, er10 ELISA Kit, inr ELISA Kit, insulin/insulin-like growth factor receptor ELISA Kit, l(3)05545 ELISA Kit, l(3)93Dj ELISA Kit, l(3)er10 ELISA Kit, lnR ELISA Kit, ir-A ELISA Kit, CTK-1 ELISA Kit, ir ELISA Kit, INSR ELISA Kit, NV14476 ELISA Kit, cd220 ELISA Kit, hhf5 ELISA Kit, insulin receptor ELISA Kit, Insulin-like receptor ELISA Kit, insulin receptor L homeolog ELISA Kit, INSR ELISA Kit, Insr ELISA Kit, InR ELISA Kit, LOC100122567 ELISA Kit, LOC100451802 ELISA Kit, insr.L ELISA Kit
    Gen-ID
    3643
    UniProt
    P06213
    Pathways
    NF-kappaB Signalweg, RTK Signalweg, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity
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