FOXO1 ELISA Kit

Produktnummer ABIN7830659

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human FOXO1.

Kurzübersicht für FOXO1 ELISA Kit (ABIN7830659)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails FOXO1 ELISA Kit

Verwendungszweck: FOXO1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FOXO1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Andere Bezeichnung: FOXO1

Hintergrund:

Synonyms: FKH1|FKHR|FOXO1A

Gene Description: forkhead box O1

Gen-ID: 2308

UniProt: Q12778

Pathways: PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling