ELK1 ELISA Kit

Produktnummer ABIN7830621

Das Human ELK1 ELISA Kit (ABIN7830621) ist ein Colorimetric ELISA Kit zur Detektion von Human ELK1.

Kurzübersicht für ELK1 ELISA Kit (ABIN7830621)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails ELK1 ELISA Kit

Verwendungszweck: ELK1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ELK1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Andere Bezeichnung: ELK1

Hintergrund:

Synonyms: -

Gene Description: ELK1, member of ETS oncogene family

Gen-ID: 2002

UniProt: P19419

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling