CDK1 ELISA Kit

Produktnummer ABIN7830490

Human CDK1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human CDK1.

Kurzübersicht für CDK1 ELISA Kit (ABIN7830490)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CDK1 ELISA Kit

Verwendungszweck: CDC2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CDC2 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: CDC2

Hintergrund:

Synonyms: CDC28A|CDK1|DKFZp686L20222|MGC111195

Gene Description: cell division cycle 2, G1 to S and G2 to M

Gen-ID: 983

UniProt: P06493

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA