CD40 ELISA Kit

Produktnummer ABIN7830485

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human CD40.

Kurzübersicht für CD40 ELISA Kit (ABIN7830485)

Target: CD40

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CD40 ELISA Kit

Verwendungszweck: CD40 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CD40 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu CD40

Target: CD40

Andere Bezeichnung: CD40

Hintergrund:

Synonyms: Bp50|CDW40|MGC9013|TNFRSF5|p50

Gene Description: CD40 Molecule, TNF receptor superfamily member 5

Gen-ID: 958

UniProt: P25942

Pathways: NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints