Caspase 9 ELISA Kit
Kurzübersicht für Caspase 9 ELISA Kit (ABIN7830473)
Target
Alle Caspase 9 (CASP9) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
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Verwendungszweck
- CASP9 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CASP9 expression in cultured cells.
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Analytische Methode
- Qualitative
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Kreuzreaktivität
- Maus, Ratte
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Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA Kit
CASP9 Reaktivität: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 Reaktivität: Ratte Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 Reaktivität: Schwein Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 Reaktivität: Maus Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 Reaktivität: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 Reaktivität: Maus Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 Reaktivität: Human cleaved Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 Reaktivität: Maus Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) ELISA KitCASP9 Reaktivität: Hund Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Uncoated
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Protokoll
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store the kit at 4°C.
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- Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))
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Andere Bezeichnung
- CASP9
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Hintergrund
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Synonyms: APAF-3|APAF3|CASPASE-9c|ICE-LAP6|MCH6
Gene Description: caspase 9, apoptosis-related cysteine peptidase
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Gen-ID
- 842
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UniProt
- P55211
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Pathways
- MAPK Signalweg, RTK Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Endopeptidase Activity
Target Alle Caspase 9 (CASP9) ELISA Kits anzeigen
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