Caspase 8 ELISA Kit

Produktnummer ABIN7830472

Human Caspase 8 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Caspase 8.

Kurzübersicht für Caspase 8 ELISA Kit (ABIN7830472)

Target: Caspase 8 (CASP8)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Caspase 8 ELISA Kit

Verwendungszweck: CASP8 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CASP8 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Caspase 8

Target: Caspase 8 (CASP8)

Andere Bezeichnung: CASP8

Hintergrund:

Synonyms: ALPS2B|CAP4|FLICE|FLJ17672|MACH|MCH5|MGC78473

Gene Description: caspase 8, apoptosis-related cysteine peptidase

Gen-ID: 841

UniProt: Q14790

Pathways: Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades