CDK1 ELISA Kit

Produktnummer ABIN6951405

Produktdetails CDK1 ELISA Kit

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reaktivität: Huhn

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Gallus CDK1/CDC2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: CDK1 (CDC2 (CDK1 Produkte)

Synonyme: CDC2 ELISA Kit, CDC28A ELISA Kit, P34CDC2 ELISA Kit, Cdc2 ELISA Kit, Cdc2a ELISA Kit, p34 ELISA Kit, cdc2 ELISA Kit, wu:fc30e01 ELISA Kit, zgc:92032 ELISA Kit, PSTAIR ELISA Kit, cdc-2 ELISA Kit, cdc2-a ELISA Kit, cdc28a ELISA Kit, cdc2x1.1 ELISA Kit, p34cdc2 ELISA Kit, xcdc2 ELISA Kit, 5363 ELISA Kit, CDCDm ELISA Kit, CDK1 ELISA Kit, CDK1/CDC2 ELISA Kit, CG5363 ELISA Kit, Cdk-1 ELISA Kit, Cdk1 ELISA Kit, Dcdc2 ELISA Kit, Dm cdc2 ELISA Kit, DmCdc2 ELISA Kit, DmCdk1 ELISA Kit, Dmcdc2 ELISA Kit, Dmel\\CG5363 ELISA Kit, cdc ELISA Kit, cdc2Dm ELISA Kit, cdk1 ELISA Kit, dCdk1 ELISA Kit, group 4 ELISA Kit, l(2)31Eh ELISA Kit, CDC2A ELISA Kit, CDC2AAT ELISA Kit, CDK2 ELISA Kit, CDKA1 ELISA Kit, CDKA;1 ELISA Kit, CYCLIN-DEPENDENT KINASE A;1 ELISA Kit, cell division control 2 ELISA Kit, cdc2-b ELISA Kit, cdc2a ELISA Kit, cdc2x1.2 ELISA Kit, POL3 ELISA Kit, pold ELISA Kit, cyclin dependent kinase 1 ELISA Kit, cyclin dependent kinase like 1 ELISA Kit, Cell division control protein 2 1 ELISA Kit, cyclin-dependent kinase 1 ELISA Kit, cyclin-dependent kinase 1 S homeolog ELISA Kit, Cyclin-dependent kinase 1 ELISA Kit, cell division control 2 ELISA Kit, cell division control protein2 homolog ELISA Kit, cyclin-dependent kinase 1 L homeolog ELISA Kit, polymerase (DNA directed), delta 1, catalytic subunit L homeolog ELISA Kit, cyclin-dependent protein kinase Cdk1/Cdc2 ELISA Kit, CDK1 ELISA Kit, CDKL1 ELISA Kit, POPTR_0004s14080g ELISA Kit, Cdk1 ELISA Kit, cdk1 ELISA Kit, cdk1.S ELISA Kit, CDC2 ELISA Kit, cdc2 ELISA Kit, cdk-1 ELISA Kit, cdk1.L ELISA Kit, pold1.L ELISA Kit

Gen-ID: 396252

UniProt: P13863

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA