TLR1 ELISA Kit

Produktnummer ABIN6720075

Hersteller Produkt- Nr.: koa0647

Dieses Human TLR1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human TLR1 zu quantifizieren.

Kurzübersicht für TLR1 ELISA Kit (ABIN6720075)

Target: TLR1 (Toll-Like Receptor 1 (TLR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails TLR1 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0647

Hersteller: Rockland

Verwendungszweck: Human TLR1 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human TLR1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TLR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TLR1 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: S22-N578

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu TLR1

Target: TLR1 (Toll-Like Receptor 1 (TLR1))

Andere Bezeichnung: TLR1

Hintergrund:

Synonyms: CD281, CD281 antigen, KIAA0012, LPRS5, rsc786, Similar to Drosophila Toll protein, TIL, TIL. LPRS5, Tlr1, TLR1_HUMAN, Toll (Drosophila) homolog, Toll like receptor 1, Toll like receptor 1 Precursor, Toll-like receptor 1, Toll/Interleukin 1 receptor like, Toll/interleukin-1 receptor-like protein

Background: TLR1 is a member of the Toll-like receptor family(TLR) of pattern recognition receptors of the innate immune system. TIR1 gene was mapped to 4p14 by fluorescence in situ hybridization. TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLR1 has also been designated as CD281(cluster of differentiation 281). TLR1 interacts with TLR2 to recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Gen-ID: 7096

NCBI Accession: NP_003254

UniProt: Q15399

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades