TLR1 ELISA Kit

Produktnummer ABIN1706080

Produktdetails TLR1 ELISA Kit

Target: TLR1 (Toll-Like Receptor 1 (TLR1))

Bindungsspezifität: AA 22-578

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TLR1

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: S22-N578

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S22-N578

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Belongs to the Toll-like receptor family.

Tissue Specificity: Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.

Plattentyp: Pre-coated

Protokoll: human TLR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TLR1 has been precoated onto 96-well plates. Standards(NSO, S22-N578) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TLR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TLR1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TLR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TLR1 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1565, Standard deviation: 84.51, CV(%): 5.4
• Sample 2: n=16, Mean(pg/ml): 3289, Standard deviation: 105.2, CV(%): 3.2
• Sample 3: n=16, Mean(pg/ml): 5057, Standard deviation: 192.2, CV(%): 3.8,
• Sample 1: n=24, Mean(pg/ml): 1833, Standard deviation: 139.3, CV(%): 7.6
• Sample 2: n=24, Mean(pg/ml): 3245, Standard deviation: 266.1, CV(%): 8.2
• Sample 3: n=24, Mean(pg/ml): 5630, Standard deviation: 444.8, CV(%): 7.9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu TLR1

Target: TLR1 (Toll-Like Receptor 1 (TLR1))

Andere Bezeichnung: TLR1 (TLR1 Produkte)

Synonyme: CD281 ELISA Kit, TIL ELISA Kit, TIL. LPRS5 ELISA Kit, rsc786 ELISA Kit, toll like receptor 1 ELISA Kit, toll-like receptor 1 ELISA Kit, TLR1 ELISA Kit, Tlr1 ELISA Kit

Hintergrund:

Protein Function: Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). .

Background: TLR1 is a member of the Toll-like receptor family(TLR) of pattern recognition receptors of the innate immune system. TIR1 gene was mapped to 4p14 by fluorescence in situ hybridization. TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLR1 has also been designated as CD281(cluster of differentiation 281). TLR1 interacts with TLR2 to recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Synonyms: Toll-like receptor 1,Toll/interleukin-1 receptor-like protein,TIL,CD281,TLR1,KIAA0012,

Full Gene Name: Toll-like receptor 1

Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Cytoplasmic vesicle, phagosome membrane, Single-pass type I membrane protein.

Gen-ID: 7096

UniProt: Q15399

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades