IL1A ELISA Kit

Produktnummer ABIN6719759

Hersteller Produkt- Nr.: koa0206

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human IL1A.

Kurzübersicht für IL1A ELISA Kit (ABIN6719759)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 4.7 pg/mL - 300 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Produktdetails IL1A ELISA Kit

Untere Nachweisgrenze: 4.7 pg/mL

Hersteller Produkt- Nr.: koa0206

Hersteller: Rockland

Verwendungszweck: Human IL-1 alpha Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human IL-1 alpha. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 0.5 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.8pg/mL, 9.4pg/mL, 4.7pg/mL human IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each human IL-1 alpha standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: S113-A271

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL-1 alpha

Hintergrund:

Synonyms: Hematopoietin 1, IFL, IFN, IFN alpha, IFN-alpha-1/13, IFNA@, IFNA1, IFNA1_HUMAN, IFNA13, IFNAP22, IL 1 alpha, Il 1a, IL1, IL1 ALPHA, IL1F1, Ilia, Interferon Alpha, Interferon alpha 1, Interferon alpha 1/13, Interferon alpha 1/13 precursor, Interferon alpha 13, Interferon alpha D, Interferon alpha leukocyte, Interferon alpha-1/13, Interferon alpha-D, Interferon leukocyte, Interleukin 1 alphar, Interleukin1 alpha, LeIF D, Preinterleukin 1, Pro interleukin 1, Pro interleukin 1 alpha, Prointerleukin 1

Background: Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18 kDa.

Gen-ID: 3552

NCBI Accession: NP_000566

UniProt: P01583

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints