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IGFBP6 IQ-ELISA Kit (Insulin-Like Growth Factor Binding Protein 6) IQ-ELISA Kit

IGFBP6 Reaktivität: Maus qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN6385615
$591.25
Zzgl. Versandkosten $45.00
96 tests
Lieferung in 11 bis 16 Werktagen
  • Target Alle IGFBP6 IQ-ELISA Kits anzeigen
    IGFBP6
    Reaktivität
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    qPCR
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Mouse Immunoquantitative (PCR-Based) IGFBP-6 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Semi-Quantitative
    Sensitivität
    0.6 pg/mL
    Produktmerkmale
    IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
    Bestandteile
    • IGFBP-6 Microplate (Item A): 96 well PCR plate coated with anti-Mouse IGFBP-6
    • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
    • Standards (Item C): 2 vials of recombinant Mouse IGFBP-6
    • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for IGFBP-6 (Item F): 2 vials of a 4x concentrated solution of anti-Mouse IGFBP-6 affinity reagent
    • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
    • Primer Solution (Item I): 1.7 ml vial
    • PCR Master Mix (Item J): 1.2 ml vial
    • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
    • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
    Benötigtes Material
    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80°C
    Featured
    Zu unserem meistverkauften IGFBP6 ELISA Kit
  • Applikationshinweise
    The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
    Kommentare

    The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

    Probenmenge
    25 µL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
    5. Add 15μL Primer solution 10μL of PCR master mix to each well
    6. Run real-time PCR
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    6 months
  • Target Alle IGFBP6 IQ-ELISA Kits anzeigen
    IGFBP6
    Andere Bezeichnung
    IGFBP-6 (IGFBP6 Produkte)
    Synonyme
    IBP6, IGFBP-6, IGF-BP6, RBP6A, IGFBP6, igfbp6, insulin like growth factor binding protein 6, insulin-like growth factor binding protein 6, IGFBP6, Igfbp6, IGFBP-6, igfbp6
    Gen-ID
    16012
    UniProt
    P47880
    Pathways
    WNT Signalweg, Myometrial Relaxation and Contraction
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