IGFBP6 ELISA Kit

Produktnummer ABIN6720227

Hersteller Produkt- Nr.: koa0592

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human IGFBP6.

Kurzübersicht für IGFBP6 ELISA Kit (ABIN6720227)

Target: IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails IGFBP6 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Hersteller Produkt- Nr.: koa0592

Hersteller: Rockland

Verwendungszweck: Human IGFBP6 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: A25-G240

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGFBP6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IGFBP6 standard solution and each sample be measured in duplicate.

Testpräzision: Intra-Assay Precision (CV%): 6.5%, 6.6%, 7%
Inter-Assay Precision (CV%): 7.8%, 7.1%, 7.9%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu IGFBP6

Target: IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))

Andere Bezeichnung: IGFBP6

Hintergrund:

Background: Insulin-like growth factor-binding protein 6 (IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.

Gene Full Name: insulin like growth factor binding protein 6

Gen-ID: 3489

NCBI Accession: NP_002169

UniProt: P24592

Pathways: WNT Signalweg, Myometrial Relaxation and Contraction