RPS6KA1 ELISA Kit

Produktnummer ABIN5658518

Produktdetails RPS6KA1 ELISA Kit

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1). No significant cross-reactivity or interference between Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) and analogues was observed.

Sensitivität: 0.117 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribosomal Protein S6 Kinase Alpha 1 (RPS6Ka1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu RPS6KA1

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Andere Bezeichnung: Ribosomal Protein S6 Kinase Alpha 1 (RPS6KA1 Produkte)

Synonyme: HU-1 ELISA Kit, MAPKAPK1A ELISA Kit, RSK ELISA Kit, RSK1 ELISA Kit, Rsk1 ELISA Kit, p90rsk ELISA Kit, rsk ELISA Kit, RPS6KA ELISA Kit, hu-1 ELISA Kit, mapkapk1a ELISA Kit, rsk1 ELISA Kit, wu:fc56h07 ELISA Kit, zgc:152654 ELISA Kit, ribosomal protein S6 kinase A1 ELISA Kit, ribosomal protein S6 kinase polypeptide 1 ELISA Kit, ribosomal protein S6 kinase A1-like ELISA Kit, ribosomal protein S6 kinase A1 L homeolog ELISA Kit, ribosomal protein S6 kinase a, polypeptide 1 ELISA Kit, RPS6KA1 ELISA Kit, Rps6ka1 ELISA Kit, RPS6KA1L ELISA Kit, rps6ka1.L ELISA Kit, rps6ka1 ELISA Kit

Hintergrund:

Gene Name: Ribosomal Protein S6 Kinase Alpha 1

Gene Aliases: HU-1, MAPKAPK1A, RSK, RSK1, S6K-alpha 1, 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, Ribosomal S6 kinase 1

Gen-ID: 6195

UniProt: Q15418

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades