RPS6KA1 ELISA Kit

Produktnummer ABIN7830975

Das Human RPS6KA1 ELISA Kit (ABIN7830975) ist ein Colorimetric ELISA Kit zur Detektion von Human RPS6KA1.

Kurzübersicht für RPS6KA1 ELISA Kit (ABIN7830975)

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails RPS6KA1 ELISA Kit

Verwendungszweck: RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu RPS6KA1

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Andere Bezeichnung: RPS6KA1

Hintergrund:

Synonyms: HU-1|MAPKAPK1A|RSK|RSK1

Gene Description: ribosomal protein S6 kinase, 90 kDa, polypeptide 1

Gen-ID: 6195

UniProt: Q15418

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades