NFkB CLIA Kit

Produktnummer ABIN5657448

Dieses Chemiluminescent ELISA kit dient der quantitativen Messung von Ratte NFkB.

Kurzübersicht für NFkB CLIA Kit (ABIN5657448)

Target: NFkB (Nuclear Factor kappa B (NFkB))

Reaktivität: Ratte

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 312.5 pg/mL - 20000 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails NFkB CLIA Kit

Untere Nachweisgrenze: 312.5 pg/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Nuclear Factor Kappa B (NFkB). No significant cross-reactivity or interference between Nuclear Factor Kappa B (NFkB) and analogues was observed.

Sensitivität: 12.56 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B (NFkB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nuclear Factor Kappa B (NFkB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nuclear Factor Kappa B (NFkB) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor Kappa B (NFkB) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor Kappa B (NFkB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: NFkB (Nuclear Factor kappa B (NFkB))

Andere Bezeichnung: Nuclear Factor Kappa B

Hintergrund:

Gene Name: Nuclear Factor Kappa B

Gene Aliases: NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit

UniProt: Q63369

Pathways: Ubiquitin Proteasome Pathway, S100 Proteine