NFkB ELISA Kit

Produktnummer ABIN416230

Ratte NFkB ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte NFkB und es wurde in 28+ Publikationen zitiert.

Kurzübersicht für NFkB ELISA Kit (ABIN416230)

Target: NFkB (Nuclear Factor kappa B (NFkB))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Produktdetails NFkB ELISA Kit

Untere Nachweisgrenze: 0.312 ng/mL

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NFkB in Tissue Homogenate,Cell Lysate,Biological Fluids

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of Nuclear Factor Kappa B (NFkB).
No significant cross-reactivity or interference between Nuclear Factor Kappa B (NFkB) and analogues was observed.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Nuclear Factor Kappa B (NFkB) and analogues was observed.

Sensitivität: < 0.114 ng/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Applikationshinweise:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor Kappa B (NFkB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor Kappa B (NFkB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Referenzen für NFkB ELISA Kit (ABIN416230)

Madi, Choucry, El-Marasy, Meselhy, El-Kashoury: "UPLC-Orbitrap HRMS metabolic profiling of Cymbopogon citratus cultivated in Egypt; neuroprotective effect against AlCl3-induced neurotoxicity in rats." in: Journal of ethnopharmacology, Vol. 259, pp. 112930, (2020) (PubMed).

Lv, Li, Hu, Wang, Wu, Li et al.: "The shear wave elastic modulus and the increased nuclear factor kappa B (NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt ..." in: Journal of biomechanics, Vol. 66, pp. 44-50, (2019) (PubMed).

Zaafan, Haridy, Abdelhamid: "Amitriptyline attenuates bleomycin-induced pulmonary fibrosis: modulation of the expression of NF-κβ, iNOS, and Nrf2." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 392, Issue 3, pp. 279-286, (2019) (PubMed).

Nahm, Nahm, Han, Gil, Choi, Lee: "Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain." in: Journal of pain research, Vol. 12, pp. 909-914, (2019) (PubMed).

George, Esmat, Tadros, El-Demerdash: "In vivo cellular and molecular gastroprotective mechanisms of chrysin; Emphasis on oxidative stress, inflammation and angiogenesis." in: European journal of pharmacology, Vol. 818, pp. 486-498, (2018) (PubMed).

Baluchnejadmojarad, Zeinali, Roghani: "Scutellarin alleviates lipopolysaccharide-induced cognitive deficits in the rat: Insights into underlying mechanisms." in: International immunopharmacology, Vol. 54, pp. 311-319, (2018) (PubMed).

Khajevand-Khazaei, Ziaee, Motevalizadeh, Rohani, Afshin-Majd, Baluchnejadmojarad, Roghani: "Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat." in: European journal of pharmacology, Vol. 826, pp. 114-122, (2018) (PubMed).

Fayed, El-Naga, Akool, El-Demerdash: "The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4." in: Drug discoveries & therapeutics, Vol. 12, Issue 2, pp. 58-67, (2018) (PubMed).

Afshin-Majd, Bashiri, Kiasalari, Baluchnejadmojarad, Sedaghat, Roghani: "Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat." in: Biomedicine & pharmacotherapy, Vol. 89, pp. 1-9, (2017) (PubMed).

Kiasalari, Heydarifard, Khalili, Afshin-Majd, Baluchnejadmojarad, Zahedi, Sanaierad, Roghani: "Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms." in: Psychopharmacology, Vol. 234, Issue 12, pp. 1841-1852, (2017) (PubMed).

Sedaghat, Taab, Kiasalari, Afshin-Majd, Baluchnejadmojarad, Roghani: "Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms." in: Biomedicine & pharmacotherapy, Vol. 87, pp. 200-208, (2017) (PubMed).

Diling, Tianqiao, Jian, Chaoqun, Ou, Yizhen: "Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus." in: Frontiers in pharmacology, Vol. 8, pp. 219, (2017) (PubMed).

Diling, Chaoqun, Jian, Jian, Jiyan, Yizhen, Guoxiao: "Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota." in: Frontiers in immunology, Vol. 8, pp. 666, (2017) (PubMed).

Abd El Motteleb, Ibrahim, Elshazly: "Sildenafil protects against bile duct ligation induced hepatic fibrosis in rats: Potential role for silent information regulator 1 (SIRT1)." in: Toxicology and applied pharmacology, Vol. 335, pp. 64-71, (2017) (PubMed).

Kiasalari, Khalili, Baluchnejadmojarad, Roghani: "Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat." in: Neurochemical research, Vol. 41, Issue 5, pp. 1065-72, (2016) (PubMed).

El-Agroudy, El-Naga, El-Razeq, El-Demerdash: "Forskolin, a hedgehog signalling inhibitor, attenuates carbon tetrachloride-induced liver fibrosis in rats." in: British journal of pharmacology, Vol. 173, Issue 22, pp. 3248-3260, (2016) (PubMed).

Kiasalari, Rahmani, Mahmoudi, Baluchnejadmojarad, Roghani: "Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation." in: Biomedicine & pharmacotherapy, Vol. 86, pp. 654-661, (2016) (PubMed).

Ahshin-Majd, Zamani, Kiamari, Kiasalari, Baluchnejadmojarad, Roghani: "Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms." in: Peptides, Vol. 86, pp. 102-111, (2016) (PubMed).

Baluchnejadmojarad, Kiasalari, Afshin-Majd, Ghasemi, Roghani: "S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase." in: European journal of pharmacology, Vol. 794, pp. 69-76, (2016) (PubMed).

Zarezadeh, Baluchnejadmojarad, Kiasalari, Afshin-Majd, Roghani: "Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms." in: European journal of pharmacology, Vol. 795, pp. 13-21, (2016) (PubMed).

Details zu NFkB

Target: NFkB (Nuclear Factor kappa B (NFkB))

Andere Bezeichnung: NFkB

Hintergrund: NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit

UniProt: Q63369

Pathways: Ubiquitin Proteasome Pathway, S100 Proteine