FOXO1 ELISA Kit
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- Target Alle FOXO1 ELISA Kits anzeigen
- FOXO1 (Forkhead Box O1 (FOXO1))
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Reaktivität
- Ratte
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.312 ng/mL - 20 ng/mL
- Untere Nachweisgrenze
- 0.312 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Forkhead Box Protein O1 (FOXO1). No significant cross-reactivity or interference between Forkhead Box Protein O1 (FOXO1) and analogues was observed.
- Sensitivität
- 0.119 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein O1 (FOXO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein O1 (FOXO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein O1 (FOXO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein O1 (FOXO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Forkhead Box Protein O1 (FOXO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Forkhead Box Protein O1 (FOXO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle FOXO1 ELISA Kits anzeigen
- FOXO1 (Forkhead Box O1 (FOXO1))
- Andere Bezeichnung
- Forkhead Box Protein O1 (FOXO1 Produkte)
- Synonyme
- FKH1 ELISA Kit, FKHR ELISA Kit, FOXO1A ELISA Kit, FoxO1a.1 ELISA Kit, foxo1 ELISA Kit, zgc:153388 ELISA Kit, Fkhr ELISA Kit, Foxo1a ELISA Kit, AI876417 ELISA Kit, Afxh ELISA Kit, Fkhr1 ELISA Kit, fkh1 ELISA Kit, fkhr ELISA Kit, foxo1a ELISA Kit, xFoxO1 ELISA Kit, FoxO1A ELISA Kit, foxo1a.2 ELISA Kit, forkhead box O1 ELISA Kit, forkhead box O1 a ELISA Kit, forkhead box O1 L homeolog ELISA Kit, forkhead box O1 b ELISA Kit, FOXO1 ELISA Kit, foxo1a ELISA Kit, Foxo1 ELISA Kit, foxo1 ELISA Kit, foxo1.L ELISA Kit, foxo1b ELISA Kit
- Hintergrund
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Gene Name: Forkhead Box Protein O1
Gene Aliases: FOX-O1, FKH1, FKHR, FOXO1A, Forkhead Homolog In Rhabdomyosarcoma
- Pathways
- PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling
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