p21 ELISA Kit

Produktnummer ABIN5653428

Produktdetails p21 ELISA Kit

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase Inhibitor 1A (CDKN1A). No significant cross-reactivity or interference between Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) and analogues was observed.

Sensitivität: 0.059 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase Inhibitor 1A (CDKN1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Andere Bezeichnung: Cyclin Dependent Kinase Inhibitor 1A (CDKN1A Produkte)

Synonyme: CCND1 ELISA Kit, CDKN1A ELISA Kit, CAP20 ELISA Kit, CDKN1 ELISA Kit, CIP1 ELISA Kit, MDA-6 ELISA Kit, P21 ELISA Kit, SDI1 ELISA Kit, WAF1 ELISA Kit, p21CIP1 ELISA Kit, CDKI ELISA Kit, Cdkn1 ELISA Kit, Waf1 ELISA Kit, mda6 ELISA Kit, p21Cip1 ELISA Kit, p21WAF ELISA Kit, Cip1 ELISA Kit, UV96 ELISA Kit, p21 ELISA Kit, cip1 ELISA Kit, p16Xic2 ELISA Kit, sdi1 ELISA Kit, waf1 ELISA Kit, cap20 ELISA Kit, cdkn1 ELISA Kit, mda-6 ELISA Kit, p21cip1 ELISA Kit, si:ch1073-48m5.2 ELISA Kit, KRAS2 ELISA Kit, p21ras ELISA Kit, cyclin D1 ELISA Kit, cyclin dependent kinase inhibitor 1A ELISA Kit, cyclin-dependent kinase inhibitor 1A (P21) ELISA Kit, cyclin-dependent kinase inhibitor 1A ELISA Kit, cyclin-dependent kinase inhibitor 1A L homeolog ELISA Kit, cyclin-dependent kinase inhibitor 1 ELISA Kit, KRAS proto-oncogene, GTPase ELISA Kit, CCND1 ELISA Kit, CDKN1A ELISA Kit, Cdkn1a ELISA Kit, cdkn1a.L ELISA Kit, cki1 ELISA Kit, cdkn1a ELISA Kit, KRAS ELISA Kit

Hintergrund:

Gene Name: Cyclin Dependent Kinase Inhibitor 1A

Gene Aliases: WAF1, p21, CIP1, p21WAF1, CAP20, CDKN1, MDA-6, SDI1, p21CIP1, CDK-Interacting Protein 1, Melanoma differentiation-associated protein 6

Gen-ID: 114851

UniProt: Q64315

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie