Cyclin A1 ELISA Kit

Produktnummer ABIN5653410

Produktdetails Cyclin A1 ELISA Kit

Target: Cyclin A1 (CCNA1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Cyclin A1 (CCNA1). No significant cross-reactivity or interference between Cyclin A1 (CCNA1) and analogues was observed.

Sensitivität: 0.049 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin A1 (CCNA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin A1 (CCNA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin A1 (CCNA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin A1 (CCNA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin A1 (CCNA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin A1 (CCNA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Cyclin A1

Target: Cyclin A1 (CCNA1)

Andere Bezeichnung: Cyclin A1 (CCNA1 Produkte)

Synonyme: fd14f08 ELISA Kit, wu:fd14f08 ELISA Kit, wu:fi20d12 ELISA Kit, zgc:111869 ELISA Kit, MGC81965 ELISA Kit, cyclin-A1 ELISA Kit, CCNA1 ELISA Kit, MGC132235 ELISA Kit, cyclin-A1-2 ELISA Kit, Cyclin-A1 ELISA Kit, ccna1 ELISA Kit, cyclin A1 ELISA Kit, cyclin A1 L homeolog ELISA Kit, CYCLIN A1;2 ELISA Kit, cyclin A1 S homeolog ELISA Kit, CCNA1 ELISA Kit, ccna1 ELISA Kit, ccna1.L ELISA Kit, CYCA1;2 ELISA Kit, Ccna1 ELISA Kit, ccna1.S ELISA Kit

Hintergrund:

Gene Name: Cyclin A1

Gene Aliases: CCN-A1

Gen-ID: 8900

UniProt: P78396

Pathways: Apoptose, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA