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CTNNB1 ELISA Kit

CTNNB1 Reaktivität: Maus Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Produktnummer ABIN5652680
  • Target Alle CTNNB1 ELISA Kits anzeigen
    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
    Reaktivität
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.62 pg/mL - 1000 pg/mL
    Untere Nachweisgrenze
    15.62 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Catenin Beta 1 (CTNNb1). No significant cross-reactivity or interference between Catenin Beta 1 (CTNNb1) and analogues was observed.
    Sensitivität
    5.9 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catenin Beta 1 (CTNNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catenin Beta 1 (CTNNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catenin Beta 1 (CTNNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catenin Beta 1 (CTNNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Beta 1 (CTNNb1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Beta 1 (CTNNb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CTNNB1 ELISA Kits anzeigen
    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
    Andere Bezeichnung
    Catenin Beta 1 (CTNNB1 Produkte)
    Synonyme
    Jup ELISA Kit, LOC100217813 ELISA Kit, CTNNB1 ELISA Kit, CTNNB ELISA Kit, Bfc ELISA Kit, Catnb ELISA Kit, Mesc ELISA Kit, ctnnb ELISA Kit, id:ibd2058 ELISA Kit, wu:fb73e10 ELISA Kit, wu:fi81c06 ELISA Kit, wu:fk25h01 ELISA Kit, ctnnb1 ELISA Kit, CHBCAT ELISA Kit, MRD19 ELISA Kit, armadillo ELISA Kit, beta-catenin ELISA Kit, catenin beta 1 ELISA Kit, catenin (cadherin associated protein), beta 1 ELISA Kit, catenin (cadherin-associated protein), beta 1 ELISA Kit, catenin beta 1 S homeolog ELISA Kit, catenin beta 1 L homeolog ELISA Kit, CTNNB1 ELISA Kit, Ctnnb1 ELISA Kit, ctnnb1 ELISA Kit, ctnnb1.S ELISA Kit, ctnnb1.L ELISA Kit
    Hintergrund

    Gene Name: Catenin Beta 1

    Gene Aliases: bCat, b-Cat, CTNN-B1, Cadherin-Associated Protein

    Gen-ID
    12387
    UniProt
    Q02248
    Pathways
    WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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