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CTNNB1 ELISA Kit

CTNNB1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6951412
  • Target Alle CTNNB1 ELISA Kits anzeigen
    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
    Reaktivität
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human CTNNB1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CTNNB1 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Haltbarkeit
    6 months
  • Target Alle CTNNB1 ELISA Kits anzeigen
    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
    Andere Bezeichnung
    CTNNB1 (CTNNB1 Produkte)
    Synonyme
    Jup ELISA Kit, LOC100217813 ELISA Kit, CTNNB1 ELISA Kit, CTNNB ELISA Kit, Bfc ELISA Kit, Catnb ELISA Kit, Mesc ELISA Kit, ctnnb ELISA Kit, id:ibd2058 ELISA Kit, wu:fb73e10 ELISA Kit, wu:fi81c06 ELISA Kit, wu:fk25h01 ELISA Kit, ctnnb1 ELISA Kit, CHBCAT ELISA Kit, MRD19 ELISA Kit, armadillo ELISA Kit, beta-catenin ELISA Kit, catenin beta 1 ELISA Kit, catenin (cadherin associated protein), beta 1 ELISA Kit, catenin (cadherin-associated protein), beta 1 ELISA Kit, catenin beta 1 S homeolog ELISA Kit, catenin beta 1 L homeolog ELISA Kit, CTNNB1 ELISA Kit, Ctnnb1 ELISA Kit, ctnnb1 ELISA Kit, ctnnb1.S ELISA Kit, ctnnb1.L ELISA Kit
    Gen-ID
    1499
    UniProt
    P35222
    Pathways
    WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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