BAX ELISA Kit

Produktnummer ABIN5652166

Produktdetails BAX ELISA Kit

Target: BAX (BCL2-Associated X Protein (BAX))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Bcl2 Associated X Protein (Bax). No significant cross-reactivity or interference between Bcl2 Associated X Protein (Bax) and analogues was observed.

Sensitivität: 26.3 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein (Bax). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Associated X Protein (Bax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated X Protein (Bax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated X Protein (Bax) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Associated X Protein (Bax) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Andere Bezeichnung: Bcl2 Associated X Protein (BAX Produkte)

Synonyme: BAX-ALPHA ELISA Kit, bax-A ELISA Kit, xBax ELISA Kit, xlbax ELISA Kit, BAX ELISA Kit, bax ELISA Kit, fj16e01 ELISA Kit, wu:fc50b10 ELISA Kit, wu:fj16e01 ELISA Kit, BCL2L4 ELISA Kit, zgc:112983 ELISA Kit, BCL2 associated X, apoptosis regulator ELISA Kit, BCL2-associated X protein L homeolog ELISA Kit, BCL2-associated X protein ELISA Kit, bcl2-associated X protein, a ELISA Kit, bcl2-associated X protein, b ELISA Kit, BAX ELISA Kit, bax.L ELISA Kit, bax ELISA Kit, baxa ELISA Kit, Bax ELISA Kit, baxb ELISA Kit

Hintergrund:

Gene Name: Bcl2 Associated X Protein

Gene Aliases: BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4

Gen-ID: 12028

UniProt: Q07813

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response