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ATF2 ELISA Kit

ATF2 Reaktivität: Human pThr69 Colorimetric Cell ELISA Adherent Cell Culture
Produktnummer ABIN5526688
  • Target Alle ATF2 ELISA Kits anzeigen
    ATF2 (Activating Transcription Factor 2 (ATF2))
    Bindungsspezifität
    pThr69, pThr71
    Reaktivität
    • 12
    • 10
    • 10
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Cell ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human Phospho-eIF-2alpha (Ser52) and Total eIF-2alpha Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures ATF2 phosphorylated at Threonine-69/71 as well as total ATF2 in adherent cell lines.
    Proben
    Adherent Cell Culture
    Analytische Methode
    Semi-Quantitative
    Spezifität
    This ELISA kit recognizes Human ATF2 phosphorylated at site Threonine-69/71 as well as total ATF2.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Bestandteile
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle ATF2 ELISA Kits anzeigen
    ATF2 (Activating Transcription Factor 2 (ATF2))
    Andere Bezeichnung
    ATF2 (ATF2 Produkte)
    Synonyme
    CRE-BP1 ELISA Kit, CREB2 ELISA Kit, HB16 ELISA Kit, TREB7 ELISA Kit, CG12850 ELISA Kit, CG3749 ELISA Kit, dATF-2 ELISA Kit, DmelCG30420 ELISA Kit, CG30420 ELISA Kit, CHN1 ELISA Kit, Atf-2 ELISA Kit, CRE-BP ELISA Kit, Creb2 ELISA Kit, D130078H02Rik ELISA Kit, D18875 ELISA Kit, Tg(Gzma-Klra1)7Wum ELISA Kit, mXBP ELISA Kit, atf2-A ELISA Kit, atf2l ELISA Kit, activating transcription factor 2 ELISA Kit, Activating transcription factor-2 ELISA Kit, activating transcription factor 2 S homeolog ELISA Kit, ATF2 ELISA Kit, Atf-2 ELISA Kit, Atf2 ELISA Kit, atf2.S ELISA Kit, atf2 ELISA Kit
    Gen-ID
    1386
    UniProt
    P15336, P16951
    Pathways
    MAPK Signalweg, RTK Signalweg, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades
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