IL1A ELISA Kit

Produktnummer ABIN5522120

Das Human IL1A ELISA Kit (ABIN5522120) ist ein Colorimetric ELISA Kit zur Detektion von Human IL1A.

Kurzübersicht für IL1A ELISA Kit (ABIN5522120)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.906 pg/mL - 250 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Produktdetails IL1A ELISA Kit

Untere Nachweisgrenze: 3.906 pg/mL

Verwendungszweck: Human IL-1α (Interleukin 1 Alpha) ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with IL-1α , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (The matrix components in serum/plasma will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing! When the content of other samples is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Healthy serum (n=18) 1/2 dilution ND-4.2pg/mL Healthy plasma (EDTA, Citrate , heparin) (n=16) 1/2 dilution ND-3.8pg/mL Human peripheral blood cells were cultured in RPMI 1640 supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate, followed by stimulated with 10 μg/mL PHA for 24 hours. The cell culture supernatants were assayed for levels of human IL-1a. 1/5-1/10 dilution 635pg/mL THP-1 cells were treated with 80nM TPA and cultured overnight.The cell culture supernatants were assayed for levels of human IL-1a. 1/2 dilution ND THP-1 cells were treated with 80nM TPA and cultured overnight. Then, stimulate with 100 ng/mL LPS for 6 hours and take the cell culture supernatant were assayed for levels of human IL-1a. 1/2 dilution 167pg/mL 2. KD sample validation (Detect IL-1α-KD THP-1 cells): Sample Type Dilution Ratio Content Wild THP-1 cells were treated with 80nM TPA and cultured overnight, and then stimulated with 200 ng/mL LPS for 6 hours. The cell culture supernatants were assayed for levels of human IL-1a. 1/2 dilution 267pg/mL THP-1 cells (+siRNA) were treated with 80nM TPA and cultured overnight, and then stimulated with 200 ng/mL LPS for 6 hours. The cell culture supernatants were assayed for levels of human IL-1a. 1/2 dilution 18pg/mL Note:ND is lower than the sensitivity of the kit and was not detected 3. Verified recombinant protein: (It is a normal phenomenon that some proteins have weak detection signals or cannot be detected at all due to differences in tags, sequences or protein activities) A. HEK293-derived Human IL-1 alpha protein Ser113-Ala271B. E. coli-derived Human IL-1 alpha protein Ser113-Ala271

Sensitivität: 2.344 pg/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Sandwich ELISA, Double Antibody

Probenmenge: 100 μL

Testdauer: 4 h

Plattentyp: Pre-coated

Protokoll: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL-1alpha

Hintergrund: Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, IL1A, IL1F1

UniProt: P01583

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints