Growth Hormone 1 ELISA Kit

Produktnummer ABIN5520678

Rind (Kuh) Growth Hormone 1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Rind (Kuh) Growth Hormone 1.

Kurzübersicht für Growth Hormone 1 ELISA Kit (ABIN5520678)

Target: Growth Hormone 1 (GH1)

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Produktdetails Growth Hormone 1 ELISA Kit

Untere Nachweisgrenze: 0.781 ng/mL

Verwendungszweck: Bovine GH (Growth Hormone) ELISA Kit

Analytische Methode: Quantitative

Spezifität: Specifically binds with GH , no obvious cross reaction with other analogues.

Sensitivität: 0.469 ng/mL

Bestandteile:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Benötigtes Material:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Competitive ELISA, Coated with Antigen

Probenmenge: 50 μL

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 50 µL standard or sample into each well. Immediately add 50 µL biotin-labeled antibody working solution into each well(The tip can't be reused after touching the liquid.), gently shake the plate for 1 min, seal closely and statically incubate for 45 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution into each well, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid direct light exposure to antibody solutions

Lagerung: 4 °C

Informationen zur Lagerung: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Haltbarkeit: 12 months

Details zu Growth Hormone 1

Target: Growth Hormone 1 (GH1)

Andere Bezeichnung: Growth Hormone

Hintergrund: Somatotropin, Growth hormone, GH1, GH

UniProt: P01246

Pathways: NF-kappaB Signalweg, JAK-STAT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process