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IGFBP6 ELISA Kit
IGFBP6
Reaktivität: Ratte
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4885876
Produktdetails IGFBP6 ELISA Kit
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Target
Alle IGFBP6 ELISA Kits anzeigen
IGFBP6
(Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))
Reaktivität
Alle Reaktivitäten für IGFBP6 ELISA Kits
Ratte
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Rat IGFBP-6 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes Rat IGFBP-6.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu IGFBP6
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Target
Alle IGFBP6 ELISA Kits anzeigen
IGFBP6
(Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))
Andere Bezeichnung
IGFBP-6 (IGFBP6 Produkte )
Synonyme
IBP6 ELISA Kit, IGFBP-6 ELISA Kit, IGF-BP6 ELISA Kit, RBP6A ELISA Kit, IGFBP6 ELISA Kit, igfbp6 ELISA Kit, insulin like growth factor binding protein 6 ELISA Kit, insulin-like growth factor binding protein 6 ELISA Kit, IGFBP6 ELISA Kit, Igfbp6 ELISA Kit, IGFBP-6 ELISA Kit, igfbp6 ELISA Kit
Gen-ID
25641
UniProt
P35572
Pathways
WNT Signalweg , Myometrial Relaxation and Contraction
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