Paxillin ELISA Kit

Produktnummer ABIN4884307

Produktdetails Paxillin ELISA Kit

Target: Paxillin (PXN)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Paxillin ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes .

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Paxillin

Target: Paxillin (PXN)

Andere Bezeichnung: Paxillin (PXN Produkte)

Synonyme: AW108311 ELISA Kit, AW123232 ELISA Kit, Pax ELISA Kit, CG18061 ELISA Kit, CG18576 ELISA Kit, CG31794 ELISA Kit, CT40481 ELISA Kit, CT42454 ELISA Kit, DPaxillin ELISA Kit, DPxn ELISA Kit, DPxn37 ELISA Kit, Dmel\\CG31794 ELISA Kit, Dpax ELISA Kit, DpaxA ELISA Kit, PDLP ELISA Kit, dPax ELISA Kit, pax ELISA Kit, wu:fw71f12 ELISA Kit, PXN ELISA Kit, LOC100220858 ELISA Kit, paxillin ELISA Kit, Paxillin ELISA Kit, paxillin S homeolog ELISA Kit, paxillin a ELISA Kit, PXN ELISA Kit, Pxn ELISA Kit, Pax ELISA Kit, pxn.S ELISA Kit, pxna ELISA Kit, LOC696336 ELISA Kit, pxn ELISA Kit

Gen-ID: 5829

UniProt: P49023

Pathways: MAPK Signalweg, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling