IL12A ELISA Kit (Interleukin 12 alpha)

Details for Product IL12A ELISA Kit No. ABIN455530, Anbieter: Anmelden zum Anzeigen
Antigen
  • IL-12p35
  • Il-12a
  • Ll12a
  • p35
  • CLMF
  • IL-12A
  • NFSK
  • NKSF1
  • P35
  • IL12p35
  • Il-12 p35
  • IL12
  • interleukin 12a
  • interleukin 12A
  • Il12a
  • IL12A
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
16
12
11
7
6
6
4
4
3
2
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
0.156-10 ng/mL
Untere Nachweisgrenze
0.156 ng/mL
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Verwendungszweck This immunoassay kit allows for the specific measurement of Mouse Interleukin,IL-12 concentrations in cell culture supernates, serum and plasma.
Proben Cell Culture Supernatant, Serum, Plasma
Analytische Methode Quantitative
Nachweismethode Colorimetric
Spezifität This assay recognizes recombinant and natural Mouse IL-12.
Kreuzreaktivität (Details) No significant cross-reactivity or interference was observed.
Produktmerkmale Mus musculus,Mouse,Interleukin-12 subunit alpha,IL-12A,Cytotoxic lymphocyte maturation factor 35 kDa subunit,CLMF p35,IL-12 subunit p35,Il12a
Plasmids, Primers & others Plasmide, Primers & weitere IL12A products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung Il12a (IL12A ELISA Kit Abstract)
Hintergrund Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of cultured EBV-transformed RPMI-8866 cells . IL-12 is a 75 kDa glycoprotein heterodimer composed of two genetically unrelated subunits linked by a disulfide bond. The smaller subunit (p35) has homology to IL-6 and G-CSF while the larger subunit (p40) demonstrates similarity to the soluble receptor for IL-6, leading to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex . Each subunit of IL-12 apparently arises from a single copy gene. The mRNA transcription of the subunits is closely coordinated, although an excess of the larger subunit has been shown to be produced by B cells in addition to active IL-12 . Expression of p35 is reported to be enhanced by simultaneous expression of p40. IL-12 activity cannot be demonstrated in the absence of either chain . As suggested by their names, p35 has a native molecular weight of 35 kDa while p40 has a native molecular weight of 40 kDa. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells . These include inducing production of IFN-gama and TNF by resting and activated T and NK cells, synergizing with other IFN-gama inducers at both the transcriptional and post-transcriptional levels to induce IFN- gama gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a comitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells . Evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells . These activities of IL-12 are antagonized by IL-4 and IL-10, factors associated with the development of uncommitted T helper cells into TH2 cells and mediation of the humoral immune response .
Pathways JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 present is bound by the immobilized antibody. An 2 enzyme-linked polyclonal antibody specific for IL-12 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Beschränkungen Nur für Forschungszwecke einsetzbar
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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