TLR4 ELISA Kit

Produktnummer ABIN424269

Das Maus TLR4 ELISA Kit (ABIN424269) ist ein Colorimetric ELISA Kit zur Detektion von Maus TLR4. Das Kit wurde in 19 Publikationen zitiert.

Kurzübersicht für TLR4 ELISA Kit (ABIN424269)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Produktdetails TLR4 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TLR4 in Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids

Analytische Methode: Quantitative

Spezifität:

This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 4 (TLR4).
No significant cross-reactivity or interference between Toll Like Receptor 4 (TLR4) and analogues was observed.

Kreuzreaktivität (Details): No significant cross-reactivity or interference between Toll Like Receptor 4 (TLR4) and analogues was observed.

Sensitivität: < 0.054 ng/mL

Bestandteile:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Benötigtes Material:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Anwendungsinformationen

Applikationshinweise:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Kommentare:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Testpräzision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handhabung: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Lagerung: 4 °C

Informationen zur Lagerung:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Haltbarkeit: 6 months

Referenzen für TLR4 ELISA Kit (ABIN424269)

Mohamadi-Zarch, Baluchnejadmojarad, Nourabadi, Khanizadeh, Roghani: "Protective effect of diosgenin on LPS/D-Gal-induced acute liver failure in C57BL/6 mice." in: Microbial pathogenesis, Vol. 146, pp. 104243, (2020) (PubMed).

Rousta, Mirahmadi, Shahmohammadi, Ramzi, Baluchnejadmojarad, Roghani: "S-allyl cysteine, an active ingredient of garlic, attenuates acute liver dysfunction induced by lipopolysaccharide/ d-galactosamine in mouse: Underlying mechanisms." in: Journal of biochemical and molecular toxicology, pp. e22518, (2020) (PubMed).

Khajevand-Khazaei, Azimi, Sedighnejad, Salari, Ghorbanpour, Baluchnejadmojarad, Mohseni-Moghaddam, Khamse, Roghani: "S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammation." in: International immunopharmacology, Vol. 69, pp. 19-26, (2019) (PubMed).

Kabel, Omar, Alhadhrami, Alharthi, Alrobaian et al.: "Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll-like receptor 4, TGF-β1, NF-κB and ..." in: Physiology & behavior, Vol. 188, pp. 108-118, (2019) (PubMed).

Fang, Xie, Liu, Fu, Ye, Liu, Zhang: "Berberine ameliorates neonatal necrotizing enterocolitis by activating the phosphoinositide 3-kinase/protein kinase B signaling pathway." in: Experimental and therapeutic medicine, Vol. 15, Issue 4, pp. 3530-3536, (2018) (PubMed).

Kabel, Abd Elmaaboud, Atef, Baali: "Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro study." in: Environmental toxicology and pharmacology, Vol. 50, pp. 216-226, (2017) (PubMed).

Qiao, He, Wei, Xia, Bao: "Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine." in: Journal of food science, Vol. 81, Issue 5, pp. H1288-94, (2016) (PubMed).

Campo, Avenoso, DAscola, Scuruchi, Calatroni, Campo: "Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytes." in: Cell and tissue research, Vol. 361, Issue 2, pp. 567-79, (2015) (PubMed).

Golec, Lemieszek, Skórska, Sitkowska, Zwoli?ski, Mackiewicz, Góra-Florek, Milanowski, Dutkiewicz: "Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in mice." in: Pathologie-biologie, Vol. 63, Issue 3, pp. 130-5, (2015) (PubMed).

Campo, Avenoso, DAscola, Scuruchi, Nastasi, Micali, Puzzolo, Pisani, Calatroni, Campo: "The SOD mimic MnTM-2-PyP(5+) reduces hyaluronan degradation-induced inflammation in mouse articular chondrocytes stimulated with Fe (II) plus ascorbate." in: The international journal of biochemistry & cell biology, Vol. 45, Issue 8, pp. 1610-9, (2014) (PubMed).

Bang, Hong, Suk, Kim, Han, Sung, Kim, Kim, Kim, Baik, Kim et al.: "Effects of Korean Red Ginseng (Panax ginseng), urushiol (Rhus vernicifera Stokes), and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) on the gut-liver axis of ..." in: Journal of ginseng research, Vol. 38, Issue 3, pp. 167-72, (2014) (PubMed).

Tun, Yasukawa, Yamada: "Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis." in: Free radical biology & medicine, Vol. 74, pp. 108-17, (2014) (PubMed).

Campo, Avenoso, DAscola, Scuruchi, Calatroni, Campo: "Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide." in: Molecular and cellular biochemistry, Vol. 399, Issue 1-2, pp. 201-8, (2014) (PubMed).

Campo, Avenoso, DAscola, Scuruchi, Prestipino, Calatroni, Campo: "6-Mer hyaluronan oligosaccharides increase IL-18 and IL-33 production in mouse synovial fibroblasts subjected to collagen-induced arthritis." in: Innate immunity, Vol. 18, Issue 5, pp. 675-84, (2013) (PubMed).

Mabalirajan, Ahmad, Rehman, Leishangthem, Dinda, Agrawal, Ghosh, Sharma: "Baicalein reduces airway injury in allergen and IL-13 induced airway inflammation." in: PLoS ONE, Vol. 8, Issue 4, pp. e62916, (2013) (PubMed).

Chang, Wang, Kao: "Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin." in: Experimental and therapeutic medicine, Vol. 6, Issue 5, pp. 1199-1207, (2013) (PubMed).

Campo, Avenoso, DAscola, Prestipino, Scuruchi, Nastasi, Calatroni, Campo: "Inhibition of hyaluronan synthesis reduced inflammatory response in mouse synovial fibroblasts subjected to collagen-induced arthritis." in: Archives of biochemistry and biophysics, Vol. 518, Issue 1, pp. 42-52, (2012) (PubMed).

Campo, Avenoso, DAscola, Scuruchi, Prestipino, Nastasi, Calatroni, Campo: "Adenosine A2A receptor activation and hyaluronan fragment inhibition reduce inflammation in mouse articular chondrocytes stimulated with interleukin-1?." in: The FEBS journal, Vol. 279, Issue 12, pp. 2120-33, (2012) (PubMed).

Fowler: "Nursing diagnosis: actual impairment of skin integrity." in: Journal of gerontological nursing, Vol. 12, Issue 10, pp. 36-7, (1986) (PubMed).

Details zu TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Andere Bezeichnung: TLR4

Hintergrund: CD284, TOLL, HToll

UniProt: Q9QUK6

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteine